Archive : Volume - 12, Issue - 12, Month - December
1 INSTRUMENTAL HANDLING AND APPLICATIONS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- Rishabh RAKESH Nimje* ,
- RANI TEKSINH BHAGAT ,
- TULSIDAS PARASRAM NIMBEKAR
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Abstract : Chromatography is described as a group of methods used to separate components in a mixture. There are two phases to this technique: stationary and movable phases. The difference in the partition coefficients of the two phases serves as the basis for the separation of constituents.
The word "chromatography" comes from the Greek words "chroma" (colour) and "graphein" (to write). For measuring pharmaceutical and environmental sample quality and quantity, high performance liquid chromatography (HPLC) is a crucial technique.
It is the most adaptable, secure, dependable, and quick chromatographic approach for determining the quality of medicinal ingredients. The analytical chemistry method of high-performance liquid chromatography (HPLC; formerly known as high-pressure liquid chromatography) is used to separate, recognise, and quantify each component in a mixture.
One type of liquid chromatography, HPLC, uses a liquid as the mobile phase. The most used kind of HPLC is reversed-phase HPLC. In a reversed-phase system, the stationary phase is relatively non-polar while the mobile phase is relatively polar. A solvent reservoir, pump, injector, column, detector, and integrator or acquisition and display system are components of HPLC instrumentation. The column where separation takes place is the brain of the system. HPLC can be used to identify, quantify, and resolve a compound, among other pieces of information.
Keyword : Chromatography, Pressure Liquid Chromatography, Types, Instrumentation, Application, Advantages & Disadvantages of HPLC
2 A Cross-Sectional study on Assessment of Quality of Life in Individuals with Generalized Anxiety Disorder
- Rama Narsimha Reddy Anreddy* ,
- A Ashwini . ,
- D Sandeep Reddy ,
- Madeeha Mahveen ,
- A Dhananjay
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Abstract : ABSTRACT
Anxiety is an emotional state that is generally caused by perception of the real or perceived danger that threatens the security of individual. The distinguish feature of generalized anxiety disorder is uncontrollable worry that lasts longer than six months and significantly impairs functioning. There is a considerable correlation between anxiety and overall quality of life, which includes social relationships, environmental wellness, psychological health, and physical health. The QOL of GAD patients is described in this study, along with the association between HAM-A and WHOQOL-BREF. The current study was a cross sectional study, the study performed by taking 120 samples. This study includes patients aged between 18-60 years and patients having GAD. This study excludes pregnant and lactating women. The peak number (85) of participants from (n=120) the study includes the age group between 20-40 years (71%). Upon distribution of data based on gender 72 (60%) were female and 48 (40%) were males. From our study majority of the patients were having moderate anxiety 40 (33.3%) when compared to other anxiety levels. From our study most of the people were experiencing a decline in QOL in psychological domain 38(31.6%), physical domain 34(28.3%), when compared to other domains. Patients from our study has significant correlation between HAM-A and WHOQOL-BREF shows that mild-moderate anxiety primarily affects the physical domain while severe-very severe anxiety significantly impacts the psychological domain. Our study included 120 patients with GAD which predominantly affects young adults aged between 20-30 years and females being more impacted than males. Our study concluded that according to the HAM-A, most patients exhibited mild-moderate anxiety levels and whereas based on WHOQOL-BREF, majority of the patients are having impairment in physical and psychological domain when compared to other domains.
Keywords: Generalized Anxiety Disorder, Quality of Life, HAM-A, WHOQOL-BREF.
Keyword : Generalized Anxiety Disorder, Quality of Life, HAM-A, WHOQOL-BREF.
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