Author Name : Triantafyllos Papadopoulos
1 In vitro evaluation of biocompatibility and cytotoxicity of Total Fill Bioceramic Root Repair material putty for endodontic use
- Triantafyllos Papadopoulos* ,
- CHARA PAPADOPOULOU ,
- MARIA GEORGOPOULOU ,
- IOANNIS KAROUSSIS ,
- KYRIAKI KYRIAKIDOU
- Abstract
- Show Article
- Download : 188
- Google Citation
- Journal DOI : 10.46624/bjmhr.2020.v7.i2.003
- Certificate
Abstract : Introduction. The purpose of this study is to evaluate the biocompatibility and cytotoxicity of Total Fill (FKG Dentaire SA, Switzerland) and compare it with white MTA and Biodentine.
Materials and methods. The biocompatibility of Total Fill (FKG Dentaire SA, Switzerland) was tested in comparison with ΜΤΑ (Tulsa Dental, Tulsa, OK) and Biodentine (Septodont, Saint Maur des Fosses, France) bioceramic materials. Cytotoxicity was investigated in terms of cell proliferation throughout the MTT assay, cell viability using FDA/PI test on MG63 pre-osteoblasts cell line for 24h, 48h and 72h. SEM micrographs have been used to describe the morphology of the cells seeded on the different ceramics. The alkaline phosphatase activity (ALP) was measured using the QUANTI-Blue method for experimental and control groups. As control the cells were seeded at the Tissue Culture Plastic (TCP). All the experiments were performed in triplicate in 3 independed runs and statistically analysed using 2-way ANOVA.
Results. MTT assay results showed a major rate of proliferation, after 72h of seeding of the MG63 cells on the Total Fill sealing material than MTA and Biodentin. These results were in accordance with those obtained by the FDA/PI double staining and the SEM micrographs.
Conclusions. All tested sealing materials performed an optimal biocompatibility, although Total Fill prevailed due to the characteristics of its solid state. In particular Total Fill presented an improved consistency which allowed the cells to populate the entire mass of the material, maintaining their viability.
Keyword : Biocompatibility, cytotoxicity, bioceramic, root canal sealer.
2 Metal-Ceramic Bond Strength of Substrates Made With Different Fabrication Techniques: In Vitro Study
- Triantafyllos Papadopoulos* ,
- Christina Mouchtaridi ,
- Marta Muñoz Hernández ,
- Konstantin Spyropoulos
- Abstract
- Show Article
- Download : 167
- Journal DOI : 10.46624/bjmhr.2020.v7.i6.011
- Certificate
Abstract : Purpose. The purpose of this in vitro study was to determine the metal-ceramic bond strength between dental porcelain and cobalt-chromium (Co-Cr) metal substrates fabricated by different techniques.
Materials and Methods. Forty Co-Cr metal substrates were fabricated according to ISO 9693-1, by casting, milling, soft milling, and DMLS. Forty additional substrates were fabricated for each technique to record the modulus of elasticity. A commercially available feldspathic porcelain was placed on the substrates, and then the specimens were also tested for metal-ceramic bond strength with the 3-point bend test, according to ISO 9693-1. The fractured specimens were observed with optical and scanning electron microscopy using electron dispersive spectroscopy to define the mode of failure. X-ray diffraction spectroscopy was conducted to determine changes in crystalline phases after fabrication and the 3-point bend test. Statistical analysis was with 1-way analysis of variance and the Tukey post hoc test (α=.05).
Results. No statistically significant differences were found for modulus of elasticity among any of the groups. The metal-ceramic bond strength for casting had no statistically significant differences and the mode of failure in all groups was cohesive. The metallographic analysis of the as-received, the after porcelain firing, and the after 3-point bend test specimens revealed changes in microstructure. The crystallographic microstructure revealed that the patterns had minor changes among the groups.
Conclusion. The study revealed that all of the techniques showed similar results. The modulus of elasticity and metal-ceramic bond strengths presented no statistically significant differences, and the mode of failure was cohesive.
Keyword : Metal-ceramic, Bond Strength, Casting, Milling, Soft Milling, DMLS, Laser Sintering
Login
Loading....
Author Guideline
Copyright Form
News Update
Archive List
- Volume-11
- Volume-10
- January 2023 Issue 1
- January 2023 Issue 1
- February 2023 Issue 2
- March 2023 Issue 3
- March 2023 Issue 3
- April 2023 Issue 4
- April 2023 Issue 4
- May 2023 Issue 5
- May 2023 Issue 5
- May 2023 Issue 5
- June 2023 Issue 6
- June 2023 Issue 6
- July 2023 Issue 7
- July 2023 Issue 7
- August 2023 Issue 8
- August 2023 Issue 8
- September 2023 Issue 9
- October 2023 Issue 10
- November 2023 Issue 11
- December 2023 Issue 12
- Volume-9
- January 2022 Issue 1
- February 2022 Issue 2
- March 2022 Issue 3
- April 2022 Issue 4
- May 2022 Issue 5
- June 2022 Issue 6
- July 2022 Issue 7
- July 2022 Issue 7
- July 2022 Issue 7
- July 2022 Issue 7
- August 2022 Issue 8
- September 2022 Issue 9
- October 2022 Issue 10
- October 2022 Issue 10
- November 2022 Issue 11
- November 2022 Issue 11
- November 2022 Issue 11
- December 2022 Issue 12
- Volume-8
- January 2021 Issue 1
- February 2021 Issue 2
- February 2021 Issue 2
- March 2021 Issue 3
- April 2021 Issue 4
- May 2021 Issue 5
- June 2021 Issue 6
- June 2021 Issue 6
- June 2021 Issue 6
- July 2021 Issue 7
- August 2021 Issue 8
- September 2021 Issue 9
- October 2021 Issue 10
- November 2021 Issue 11
- December 2021 Issue 12
- Volume-7
- January 2020 Issue 1
- January 2020 Issue 1
- February 2020 Issue 2
- March 2020 Issue 3
- March 2020 Issue 3
- March 2020 Issue 3
- April 2020 Issue 4
- April 2020 Issue 4
- May 2020 Issue 5
- May 2020 Issue 5
- May 2020 Issue 5
- June 2020 Issue 6
- June 2020 Issue 6
- June 2020 Issue 6
- July 2020 Issue 7
- August 2020 Issue 8
- September 2020 Issue 9
- October 2020 Issue 10
- November 2020 Issue 11
- December 2020 Issue 12
- December 2020 Issue 12
- Volume-6
- January 2019 Issue 1
- February 2019 Issue 2
- March 2019 Issue 3
- April 2019 Issue 4
- April 2019 Issue 4
- May 2019 Issue 5
- May 2019 Issue 5
- June 2019 Issue 6
- June 2019 Issue 6
- July 2019 Issue 7
- July 2019 Issue 7
- August 2019 Issue 8
- September 2019 Issue 9
- September 2019 Issue 9
- September 2019 Issue 9
- October 2019 Issue 10
- November 2019 Issue 11
- November 2019 Issue 11
- November 2019 Issue 11
- December 2019 Issue 12
- Volume-5
- January 2018 Issue 1
- January 2018 Issue 1
- February 2018 Issue 2
- March 2018 Issue 3
- April 2018 Issue 4
- May 2018 Issue 5
- June 2018 Issue 6
- July 2018 Issue 7
- August 2018 Issue 8
- September 2018 Issue 9
- September 2018 Issue 9
- October 2018 Issue 10
- October 2018 Issue 10
- November 2018 Issue 11
- December 2018 Issue 12
- December 2018 Issue 12
- Volume-4
- January 2017 Issue 1
- February 2017 Issue 2
- February 2017 Issue 2
- March 2017 Issue 3
- April 2017 Issue 4
- April 2017 Issue 4
- May 2017 Issue 5
- June 2017 Issue 6
- June 2017 Issue 6
- June 2017 Issue 6
- June 2017 Issue 6
- July 2017 Issue 7
- August 2017 Issue 8
- August 2017 Issue 8
- September 2017 Issue 9
- October 2017 Issue 10
- November 2017 Issue 11
- December 2017 Issue 12
- December 2017 Issue 12
- December 2017 Issue 12
- Volume-3
- January 2016 Issue 1
- February 2016 Issue 2
- March 2016 Issue 3
- April 2016 Issue 4
- May 2016 Issue 5
- June 2016 Issue 6
- June 2016 Issue 6
- July 2016 Issue 7
- July 2016 Issue 7
- July 2016 Issue 7
- August 2016 Issue 8
- September 2016 Issue 9
- October 2016 Issue 10
- October 2016 Issue 10
- November 2016 Issue 11
- November 2016 Issue 11
- December 2016 Issue 12
- Volume-2
- Volume-1
Statastics
Download of Articles
115,510