Validation.

A simple, precise and rapid HPLC method has been developed for the simultaneous determination of Indapamide and Perindopril in pharmaceutical dosage form. The method was carried out using Hypersil BDS C18 column (250 mm x 4.6 mm, 5µm) and mobile phase of phosphate buffer pH 3.5±0.05 and methanol in the ratio of 65:35 v/v and degassed under ultrasonication. The flow rate was 1.0 mL/min and the effluent was monitored at 215 nm. The retention times of Perindopril and Indapamide were 3.53 min and 4.09 min respectively. The method was validated. Linearity was in the range of 160 to 480 µg/mL for Perindopril and 50 to 150 µg/mL for Indapamide respectively. The percentage recoveries of both the drugs were ranging from 97.8 to 101.7 for Perindopril and 98.7 to 101.8 for Indapamide respectively from the tablet formulation. The proposed method is suitable for the routine quality control analysis of simultaneous determination of Perindopril and Indapamide in pharmaceutical dosage form.

A simple, rapid, precise, accurate and sensitive reverse phase high performance liquid chromatography(RP-HPLC) method for simultaneous estimation of Pantoprazole and Domperidone in pharmaceutical dosage forms have been developed and validated. Drug was resolved on a C18 column (Phenomenex phenyl hexyl column, 250mm*4.6mmi.d,5um). Utilizing mobile phase of water with 0.4%v/v triethyl amine and acetonitrile with diluted orthophosphoric acid pH adjusted to 5.2 in a ratio of 50:50 of water and acetonitrile respectively. Mobile phase was delivered at the flow rate of 1.0ml/min. Ultraviolet detection was carried out at 236nm. Separation was completed within 7.75 minutes. Calibration curve was linear with correlation coefficient (r2) =0.999. Using etoricoxib 10ug/ml as IS. Recovery was between 99.26, 100.2 percentage. The standard deviation was found to be less than 1% for the assay of tablet.. The proposed methods were successfully employed for the estimation of Pantoprazole and Domperidone in combined tablet formulation.

Sildenafil Citrate (50mg) and Dapoxetine Hydrochloride (30mg) in combination used for the treatment of Premature ejaculation and Erectile dysfunction. Market survey and literature review revealed that there were so many methods available for single Sildenafil Citrate and DapoxetineHydrocloride and UV spectrophotometric method by absorbance correction method and simultaneous equation method (vierodt’s method) are available in 2013 . So it was thought of intrest to develop and validate simple, accurate, sensitive and rapid new UV spectrophotometric and RP-HPLC method for simultaneous estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in Pharmaceutical dosage form. In RP-HPLC method, chromatographic separation was achieved on Hypersil ODS C18 (250mm x 4.6 mm id, 5m particle size) column using Methanol:Water buffer at pH 3 (pH of buffer was adjusted to 3.0 with dilute 0.5% ortho-phoshporic acid) (95:05 v/v) as mobile phase. Detection was carried out at 225 nm.