Diffusion assay

Gels are transparent to opaque semisolid containing gelling agent that merges or entangles to form a three dimensional colloidal network structure. Its responsible for a gel resistance to deformation and its visco-elastic properties. Gels have better potential as a vehicle to administer rug topically in comparison to ointments because they are non-sticky, require low energy during formulation, have aesthetics value and are stable. The physical appearance was simply checked by colour, odour, taste. In this a homogeneity and texture of prepared gel was also determined. Viscosity was determine using Brookfeild digital viscometer. 5gm prepared gel sample was placed in sample holder of B. Viscometer using spindle no.6 at 25 0c and allowed it to settle for 5 min and viscosity measured by rotating it at different rpm. Antiheadlice activity was determined by using antibacterial method by diffusion assay or cylinder plat or cup- plate method.