Collagen

OBJECTIVE: The aim of the study was to analyse and compare the nature of stromal collagen fibers in different grade of Oral Squamous Cell Carcinoma(OSCC) by using different connective tissue stains like Masson’s Trichrome ,Van Geison and Picrosirius stains and to understand the possible role of stroma in progression of OSCC MATERIALS AND METHOD: Total 44 cases of OSCC and 5 sections of controls were examined using three connective tissue special histochemical stains Results: In this study, we found a gradual change in polarizing colours of collagen fibers from orange red to yellowish green from well to poorly differentiated OSCC around the tumour islands Conclusion: It was observed that stromal changes at the invading front of tumour islands and with the progression of neoplasm can be evaluated more efficiently with the use of Picrosirius stain, Masson Trichrome stain and van Geison stain

Background: Diabetes mellitus is a common serious metabolic disorder with well-known serious secondary complications. Long term hyperglycemia induced- neurotoxicity leads to many adverse effects on various parts of both central and peripheral nervous system. The mechanisms responsible for the diabetes-related neuro-chemical alteration and structural abnormalities in the brain are not fully understood as yet. Aim: The aim of this current experimental study was to carry out a histological and histomorphological analysis of the diabetes-related changes in the cerebellar cortex of STZ-induced diabetic rats. Materials and methods: 36 Albino rats weighing approximately 250 g were included in the study and divided in to control and diabetic groups and each group having 6 rats. Diabetic group received single dose of streptozotocin (60 mg/kg/bw, i.p.) and control animals received normal saline. The induction of diabetes was confirmed by measuring the blood glucose levels from the lateral tail blood and blood sugar level above 250 mg/dL were considered diabetic. After experimental period all groups’ rats were sacrificed and coronal sections were taken from the cerebellum and stained with Cresyl violet, LFB, and PSR. The number of Purkinje and thickness of different layers of the cerebellum was evaluated for histomorphometry. Results: Light microscopic studies and biochemical estimation revealed that there is reduction of number as well as diameter of Purkinje cells and reduced thickness of molecular and granular layer of cerebellar cortex. There is progressive increase in the amount of collagen fibers around tunica adventitia of cerebellar cortical as well as medullary vessels and choroid plexus of fourth ventricle. Alteration of biochemical changes in the form of increased serum creatinine level and decreased serum total protein was also noticed with increasing duration of hyperglycemia. Conclusion: It is therefore concluded that long-standing hyperglycemia leads to reduction of number Purkinje cells and thickness of cerebellar cortical layers; increased collagen around cerebellar vessels and choroid plexus in conjunction with the biochemical changes appear to promote the cerebellar functional alterations observed in chronic diabetics.