Pseudomonas aeruginosa

Pseudomonas aeruginosa is the most common Gram-negative bacteria that causes fatal nosocomial infections amongst hospitalized patients, especially immunocompromised subjects. The bacteria resist a wide variety of currently available antibiotics. One of the resistant mechanisms includes forming biofilms in the infected host, which prevents the accessibility of drugs to their respective bacterial cell targets. A novel strategy to overcome this antibiotic resistance mechanism is the use of antibiotics in combination with biofilm formation inhibitors. The objective of this study was to investigate the effect of mulberry-leaf extracts on P. aeruginosa biofilm formation. The results of specific biofilm formation (SBF) index evaluations showed that mulberry-leaf ethanolic (256 g/ml) and ether extracts (32 g/ml) presented strong capacities (SBF index > 1.1) to reduce biofilm formation by 44.0% and 44.8%, respectively. Each plant extract anti-biofilm activity was tested further in combination with gentamicin (0.125 to 1,024 µg/mL) using the checkerboard method; however, no synergistic efficacy was observed. The MBEC and CLSM results revealed that the ether extract (32 g/ml) was effective against P. aeruginosa biofilm formation. This study should provide an alternative strategy for the effective treatment of deadly infections caused by the most common drug resistant bacteria, P. aeruginosa.

Biological synthesis of nanoparticles has received increased attention due to a growing need to develop environmentally benign technologies in material synthesis. In present work silver nanoparticles (AgNps) were synthesized intracellular by using Pseudomonas aeruginosa sp. The silver nanoparticles were characterized by Transmission Electron Microscopy (TEM), UV-Visible Spectroscopy (UV). Larvicidal activities of silver nanoparticles were analyzed as per the standard procedures. The Pseudomonas aeruginosa synthesized AgNPs were found effective against the larvae and pupae of Cx. quinquefasciatus. The larvaes of Cx. quinquefasciatus were found highly susceptible to the synthesized AgNPs at the same test concentrations. The mortality could be observed after different hours of exposure. The present suggested that the bacterial mediated AgNPs can be use to kill larva, pupa of filarial vector and could be significantly reduce parasite transmission and therefore lead to reduced filarial risk.