Ganoderma lucidum

Ganoderma lucidum, basidiomycetes fungus critically involved in cancer and neurodegenerative diseases involving myco-constituents majorly by terpenoids, polysaccharides and proteins. Revealing and delving deeper mechanism in the proteomics brings out numerous protein sequence known as hypothetical proteins. One such protein in Ganoderma lucidum is Galu_Mp19 with 192 amino acids whose homology model is prepared for the first time and structurally assigned as homing endonuclease LAGLIDADG type of protein. The model was evaluated by various bioinformatics tools and the conserve domain residues of the protein, revealed by the comparative sequence analysis, were investigated by the different protein analysis tool. Residues ranging from 57-156 are conserved sequences in the LAGLIDADG in which Arg70, Ile71, Lys73, Gly80, Met122, Tyr123, Gln146, Ser180, Ala184, Lys185 and Arg186 residues exhibits top energy model during DNA-protein binding prediction. Prominently, LAGLIDADG engaged in genome analysis, gene manipulation, cloning, recombination events, DSB repair and transposition as rare-cutting endonucleases to uphold chromosomal integrity and viability.