Poosarla Aparanji

Abstract Psoriasis is a common inflammatory skin disease with an incompletely understood etiology. The disease is characterized by red, scaly and well-demarcated skin lesions formed by the hyperproliferation of epidermal keratinocytes. This hyperproliferation is driven by cytokines secreted by activated resident immune cells, an infiltrate of T cells, dendritic cells and cells of the innate immune system, as well as the keratinocytes themselves. Psoriasis has a strong hereditary character and has a complex genetic background. Genome-wide association studies have identified polymorphisms within or near a number of genes encoding cytokines, cytokine receptors or elements of their signal transduction pathways, further implicating these cytokines in the psoriasis path mechanism. A considerable number of inflammatory cytokines have been shown to be elevated in lesional psoriasis skin, and the serum concentrations of a subset of these also correlate with psoriasis disease severity. The combined effects of the cytokines found in psoriasis lesions likely explain most of the clinical features of psoriasis, such as the hyperproliferation of keratinocytes, increased neovascularization and skin inflammation. Thus, understanding which cytokines play a pivotal role in the disease process can suggest potential therapeutic targets. A number of cytokines have been therapeutically targeted with success, revolutionizing treatment of this disease. Here we review a number of key cytokines implicated in the pathogenesis of psoriasis.

In the present study we focused on effect of ethyl acetate fraction purified from stylissa carteri a marine sponge collected from Andaman on human Blood PBMC and skin keratinocytes from psoriasis patients. The ethyl acetate fraction of stylissa carteri, a marine sponge collected from Andaman and nicobar islands, was found to exhibit decreased human skin keratinocyte count and human PBMC count in Blood. We also observed the elevation of keratinocyte count as well as PBMC count in various in vitro mitogens such as Con A, PHA, PMA induced cell proliferative responses of psoriatic humans. The ethyl acetate fraction of stylissa carteri, which then inhibits the immune mediated skin destruction, which occurs in the pathogenesis of psoriatic patients. However, the ethyl acetate fraction of stylissa carteri In vito treatment of psoriatic humans skin cells and PBMC reduced considerably the increase of cell proliferative response comparative to the normal levels. Human PBMC are proven to be valuable research programmed by the study of pathogenic mechanism of this diseases as well as for testing new therapies