Omer F Idri

Assessment of serum liver enzyme and trace element levels was carried out in a total of hundred (100) subjects comprising seventy (70) sickle cell disease patients attending Madani learning hospital comparatively with thirty (30) apparently healthy persons as control subjects. Blood samples were collected from participants, they were analyzed for trace elements using atomic absorption spectrophotometer. The enzymes activities for serum glutamic oxaloacetic transamineses (SGOT), serum glutamic pyruvic transaminases (SGPT), and Alkaline phosphatase (ALP) were measured using the kinetic tests depending on the reaction with the substrates (2.4-dinitrophenylhydrazine and P. Nitro phenyl). The mean serum level of Magnesium, Zinc, in sickle cell disease patients were 13.1000 ± 1.89 mg/L, 0.40883 ± 0.095 mg/L respectively . Serum magnesium, zinc levels were significantly lower (p<0.05) in sickle cell disease patients when compared with the control. While liver enzymes were in a significant difference (P<0.01). SGOT reported low activity while ALP reported higher activity between the study groups. Serum trace elements levels was not age or sex dependent, as similar pattern of serum trace elements was observed in both male and female sickle cell disease patients. The age and sex distribution for SCD patients indicated tremendous increases in the number and percentage of the disease among males and females in the age between 2-9 years (66.7%, 57.5%) respectively. Seventy (70) patients with homozygous sickle cell (SS) disease, with both sexes. Their age ranged between 6 months to 17 years. Thirty persons (30) were used as control. Freshly obtained blood samples from patients and control were used to estimate biochemical parameters which include; liver function test (SGOT & SGPT and alkaline phosphatase), and trace element (Zinc, Magnesium). Different analytical (colorimetri, spectrophotomer) methods were used to determine biochemical parameters indicated above. Serum tracee

This is a case control study. It was conducted at Madani educational Hospital in Aljazeera state. The aim of the present study is to evaluate Serum urea, creatinine, Na, K, copper and selenium levels in sickle cell disease patients. A total of 70 sickle cell disease patients were enrolled in this study, in addition to 30 healthy’ children as a control group age and sex matched apparently healthy control subjects. Blood samples collected from participants were analyzed for trace elements using atomic absorption spectrophotometer. The results of the present study showed a significant increase in the mean of serum urea levels in patients when compared with control group (58.76±10.19) versus (34.27±7.13) mg/dl respectively with (P-value 0.00). Result of creatinine indicated insignificant increase when compared mean of patients with mean of control group (0.87±0.22) versus (0.42±0.07) mg/dl with (P-value 0.07). Also there was insignificant difference in the serum Na and K in mean of patients comparing with mean of control group (138.77±6.47) versus (139.5±2.93)m.mol/l (P-value 0. 5), (4.28±0.74) versus (3.91±0.34) m. mol/l (P-value 0.10). In the current study there was significant increase in the mean of serum copper levels when compared mean of patients with mean of control group(1.61±0.22) versus (1.03±0.23) mg/ L (P-value 0.00) but in the mean of serum selenium levels there was a significant decrease(64.91 ± 3.780) versus (85.40±8.82) (P-value 0.00). Conclusively, assessment of trace element levels is vital in the management of sickle cell disease. and thereby improving the chances of survival in sickle cell disease.