Partial Purification of Extracellular Lipase from Soil Fungal Isolate

Lipases are biotechnologically vital cluster of enzymes reported to be produced by many species of animals, plants, bacteria, yeasts and fungi. Lipases of microbial origin that are employed in the detergent, dairy, cosmetic, food, and tanning industries are mostly engaging. The present study was aimed at partial purification of lipase produced from soil fungal isolate. Hyper producer strain was selected by growing fungal isolate onto the tributyrin agar media plates. Extracellular lipase was produced by inoculating the isolated strain in fermentation broth followed by incubation at 28ºC for 5 days at 120rpm. Mycelium free culture supernatant obtained after centrifugation of fungus broth was used for purification study. Among the ammonium sulphate precipitates, 60% fractionate showed the highest lipase activity and protein content, and on further dialysis the lipase activity was increased. 2.78 fold purification was obtained by 60% ammonium sulphate dialyzed precipitation which is higher than the fold purification of crude lysate.