Colorectal cancer one of the mostly found cancer in all over the world. Mortality rate by this cancer is high compared to other types o cancers. Early diagnosis of this cancer is required to treat this cancer. Many drugs are being used to treat this disease but they all have many side effects so in study we tried to overcome this issue by treating this cancer with supplements. We used male albino SD rats to conduct this study. We divided the rats into 7 groups in which 4 of them were given Azoxymethane (AOM) to induce the colo-rectal cancer while remaining 3 were kept control. One group with AOM was not given any treatment while 3 received folic acid dose (20, 40, and 60) respectively along with Vitamin A, C, and E supplementation. Control groups without AMO were also treated with same doses of folic acid in order to compare the results. Results reveal that Folic acid improved GSH, TAC, Lipid peroxidation and superoxide dismutase levels in the AMO treated groups. These tests were also improved in control groups with folic acid and vitamin supplementation. We conclude that Folic acid is found very affective against the colorectal cancer without harming the natural mechanisms in the body. The aim of this study, to evaluate the preventive and protective measure of different doses of folic acid with normal therapeutic doses of vitamin A, C and E against azoxymethane induce colon cancer in rat.