Background: Poor semen liquefaction is one of the causes of male infertility which has become a public health challenge in Nigeria. Abnormal sperm motility one of the commonest abnormalities detected in infertile males. The pathogenesis of poor liquefaction and solidification of semen are modulated by Semenogelin and some other secretions from male accessory organs. Objective: To evaluate the concentrations of Semenogelin and zinc in seminal plasma and to correlate the levels with percentage sperm motility. Materials and methods: Four hundred men, mean age 36.4±6.8 years who were evaluated for infertility and 100 men, mean age 40.2±6.6 years of proven fertility were consecutively recruited for the study. Following routine semen analysis, seminal plasma Semenogelin-1 and zinc were determined by ELISA technique and Atomic Absorption spectrophotometry techniques respectively. Data generated were compared between groups and association between Semenogelin, zinc and percentage sperm motility was determined. Results: Seminal plasma Semenogelin level was higher (p