Biological synthesis of nanoparticles has received increased attention due to a growing need to develop environmentally benign technologies in material synthesis. In present work silver nanoparticles (AgNps) were synthesized intracellular by using Pseudomonas aeruginosa sp. The silver nanoparticles were characterized by Transmission Electron Microscopy (TEM), UV-Visible Spectroscopy (UV). Larvicidal activities of silver nanoparticles were analyzed as per the standard procedures. The Pseudomonas aeruginosa synthesized AgNPs were found effective against the larvae and pupae of Cx. quinquefasciatus. The larvaes of Cx. quinquefasciatus were found highly susceptible to the synthesized AgNPs at the same test concentrations. The mortality could be observed after different hours of exposure. The present suggested that the bacterial mediated AgNPs can be use to kill larva, pupa of filarial vector and could be significantly reduce parasite transmission and therefore lead to reduced filarial risk.