Diabetes disease is a serious and costly health problem, particularly in developing countries where the medication is unavailable for all. Leaves of Moringa oleifera are used in Sudanese traditional medicine as anti- diabetic. This study aimed to evaluate this antidiabetic property on rats. Methods: aqueous leaf extract of Moringa oleifera was administered to STZ-induced rats representing type-2 and type-1 diabetes. Then, body weighting, fasting blood glucose (FBG), intravenous Glucose tolerance test (GTT) was evaluated in treated and untreated rats. Besides, the phytochemical screening of the plant. Results: Moringa oleifera leaves showed anti-diabetic properties, being rich in phytochemicals of medical properties, supporting the traditional use. Conclusion: This edible plant is recommended as a food supplement for diabetic patients.