British Journal of Medical and Health Research BJMHR 2394-2967 BJMHR0202002 The Sensitivity of Gram +ve, Gram -ve Bacterial Pathogens to the Antibacterial Activity of Cultured Tissues Croton Bonpladianum Baill and their Phytochemical Studies Saripalli Harikrishna R Nandam Lydia S Prasad Madan Department Of Biotechnology, College Of Natural And Computational Sciences, Aksum University, Axum, Ethiopia, P.O.Box:1010, North East Africa Department Of Biotechnology, R.P.S. College (M.U), 824 234, Patna, North India 02 01 2015 2 2

The different fractions of methanolic extracts of cultured tissues of Croton bonplandianum Bail were subjected to preliminary phytochemical and in-vitro anti – bacterial studies. The different fractions revealed the presence of steroids, alkaloids, flavonoids and saponins. The antibacterial activity of the plant (Callus Cultures) different fractions of methanolic extracts was assayed by the agar plate disc diffusion technique. Three gram positive, four gram negative bacterial species were screened for the anti-bacterial investigations. The fraction II of the methanolic extract inhibited the growth of all the test bacterial species whereas fraction III and fraction IV have shown weak antibacterial activity.

 Antibacterial Cultured tissues Croton bonplandianum Baill and Phytochemical.