This study was carried out to analyze and compare the concentration of vitamin D in the metabolic syndrome's individuals (diabetics, hypertensive, obese) with normal individuals. The study involved a group of healthy individuals as controls (N = 95, age range was 18-68 years) matched with a test group of metabolic syndrome's individuals (N = 65, age range was 11-61years). The groups contain 130 female and 130 male. The serum 25-OH vitamin D level was estimated using ELISA method. Appropriate statistical tests were used to assess significant difference in the means of the estimated concentrations between patients and the control group. The mean serum level of vitamin D significantly lower in metabolic syndrome's groups (diabetics: 20.32±12.46 ng/ml, hypertensive: 24.50±10.69 ng/ml, obese: 21.60±6.75 ng/ml) when compared with control group (37.52±11.14 ng/ml), P< 0.000. It may be useful to do early screening and treatment of hypovitaminosis D in metabolic syndrome's individuals to prevent the vitamin D deficiency and its complications.