Sildenafil Citrate (50mg) and Dapoxetine Hydrochloride (30mg) in combination used for the treatment of Premature ejaculation and Erectile dysfunction. Market survey and literature review revealed that there were so many methods available for single Sildenafil Citrate and DapoxetineHydrocloride and UV spectrophotometric method by absorbance correction method and simultaneous equation method (vierodt’s method) are available in 2013 . So it was thought of intrest to develop and validate simple, accurate, sensitive and rapid new UV spectrophotometric and RP-HPLC method for simultaneous estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in Pharmaceutical dosage form. In RP-HPLC method, chromatographic separation was achieved on Hypersil ODS C18 (250mm x 4.6 mm id, 5m particle size) column using Methanol:Water buffer at pH 3 (pH of buffer was adjusted to 3.0 with dilute 0.5% ortho-phoshporic acid) (95:05 v/v) as mobile phase. Detection was carried out at 225 nm.